irf1 d5e4 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
irf1 d5e4
Irf1 D5e4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+irf1/pmc13054447-167-9-19
Average 86 stars, based on 1 article reviews
Irf1 D5e4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+irf1/pmc13054447-167-9-19
Average 86 stars, based on 1 article reviews
irf1 d5e4 - by Bioz Stars,
2026-10
86/100 stars
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Nucleic Acid Electrophoresis:Article Title: Targeting PTPN13 with 11-amino-acid peptides of C-terminal APC prevents immune evasion of colorectal cancer Article Snippet: Protein concentration was determined using a BCA assay kit (#23227, Thermo Fisher Scientific). .. Cell lysates were separated by 4%–5% Tris-glycine gel electrophoresis, transferred to polyvinylidene difluoride membranes (#HVHP29325 or #GVHP29325, Millipore), and incubated overnight at 4 °C with the appropriate primary antibodies: anti-APC (1:100 dilution, #ab15270, abcam), anti-Phospho-JAK1 (1:100, #3332S, Cell Signaling Technology), anti-JAK1 (1:100, #3331S, Cell Signaling Technology), anti-Phospho-STAT1 (1:100, #9167S, Cell Signaling Technology), anti-STAT1 (1:500, #9172S, Cell Signaling Technology), Incubation:Article Title: Targeting PTPN13 with 11-amino-acid peptides of C-terminal APC prevents immune evasion of colorectal cancer Article Snippet: Protein concentration was determined using a BCA assay kit (#23227, Thermo Fisher Scientific). .. Cell lysates were separated by 4%–5% Tris-glycine gel electrophoresis, transferred to polyvinylidene difluoride membranes (#HVHP29325 or #GVHP29325, Millipore), and incubated overnight at 4 °C with the appropriate primary antibodies: anti-APC (1:100 dilution, #ab15270, abcam), anti-Phospho-JAK1 (1:100, #3332S, Cell Signaling Technology), anti-JAK1 (1:100, #3331S, Cell Signaling Technology), anti-Phospho-STAT1 (1:100, #9167S, Cell Signaling Technology), anti-STAT1 (1:500, #9172S, Cell Signaling Technology), Article Title: Tumoral ALOX5 mediated arachidonic acid metabolism regulates immune response in non-small cell lung cancer Article Snippet: Equal amounts of protein were mixed with loading buffer and heated at 95 °C for 10 min. Proteins were separated by SDS-PAGE, transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore), and then blocked with 5% non-fat milk in TBST (TBS + 0.1% Tween 20) at room temperature for 1.5h. .. The membrane was incubated overnight at 4 °C with primary antibodies, including anti-ALOX5, sc-136195, santa-cruz; anti-β-Actin, sc-47778, santa curz; anti-Flag, F1408, Sigma; anti-p-JAK1, 74129, CST; anti-pSTAT1, 8826, CST; Article Title: AIM2 drives inflammatory cell death and monkeypox pathogenesis Article Snippet: After electrophoretic transfer onto PVDF membranes (Millipore, IPVH00010), nonspecific binding was blocked with 5% skim milk. .. The membranes were subsequently incubated with the following primary antibodies: anti-caspase-1 (AdipoGen, AG-20B-0042, 1:2000), anti-caspase-3 (CST, #9662, 1:2000), anti-cleaved caspase-3 (CST, #9661, 1:2000), anti-caspase-7 (CST, #9492, 1:2000), anti-cleaved caspase-7 (CST, #9491, 1:2000), anti-caspase-8 (CST, #4927, 1:2000), anti-cleaved caspase-8 (CST, #8592, 1:2000), anti-pMLKL (CST, #37333, 1:2000), anti-MLKL (Abgent, AP14272b, 1:2000), anti-GSDMD (Abcam, ab209845, 1:2000), anti-pRIPK3 (CST, 91702 S, 1:2000), anti-RIPK3 (ProSci, 2283, 1:2000), Article Title: MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer Article Snippet: The protein was loaded into the wells of a 12% SDS-PAGE gel (BioFroxx, China) (40 μg/well) and separated at 80 V for 30 minutes, followed by 120 V until the protein was fully resolved. .. The proteins were transferred onto a PVDF membrane, which was then blocked for 15 minutes using a blocking solution and incubated overnight at 4 °C with the following primary antibodies: Anti-pro-caspase-1 (1:1000, ab179515, Abcam, United Kingdom), anti-GSDMD (1:1000, ab210070, Abcam, United Kingdom), anti-cleaved caspase-3 (1:500, ab32042, Abcam, United Kingdom), anti-cleaved caspase-7 (1:1000, ab256469, Abcam, United Kingdom), anti-cleaved caspase-8 (1:1000, #98134, CST, United States), anti-RIPK1 (1:1000, AB300617, Abcam, United Kingdom), anti-p-RIPK1 (1:1000, ab316923, Abcam, United Kingdom), anti-MLKL (1:2000, ab184718, Abcam, United Kingdom), anti-p-MLKL (1:1000, ab187091, Abcam, United Kingdom), anti-GSDME-N (1:1000, ab215191, Abcam, United Kingdom), Article Title: SLC5A11 Mediates Metformin-Induced PD-L1 Suppression to Enhance Cancer Immunotherapy through AMPK-IRF1 Signaling. Article Snippet: 3 Metformin exhibits immunomodulatory properties in cancer treatment, but the 4 underlying mechanisms remain elusive.. Using genome-wide CRISPR screening, we 5 identified SLC5A11 as an essential mediator of metformin sensitivity.. Molecular 6 docking and dynamics simulations revealed direct metformin-SLC5A11 binding at the 7 pocket containing Asn78 and Glu102 residues. Article Title: AIM2 drives inflammatory cell death and monkeypox pathogenesis. Article Snippet: After electrophoretic transfer onto PVDF membranes (Millipore, IPVH00010), nonspecific binding was blocked with 5% skim milk. .. The membranes were subsequently incubated with the following primary antibodies: anti-caspase-1 (AdipoGen, AG-20B0042, 1:2000), anti-caspase-3 (CST, #9662, 1:2000), anti-cleaved caspase-3 (CST, #9661, 1:2000), anti-caspase-7 (CST, #9492, 1:2000), anti-cleaved caspase-7 (CST, #9491, 1:2000), anti-caspase-8 (CST, #4927, 1:2000), anti-cleaved caspase-8 (CST, #8592, 1:2000), antipMLKL (CST, #37333, 1:2000), anti-MLKL (Abgent, AP14272b, 1:2000), anti-GSDMD (Abcam, ab209845, 1:2000), anti-pRIPK3 (CST, 91702 S, 1:2000), anti-RIPK3 (ProSci, 2283, 1:2000), Article Title: Tumoral ALOX5 mediated arachidonic acid metabolism regulates immune response in non-small cell lung cancer. Article Snippet: Equal amounts of protein were mixed with loading buffer and heated at 95 °C for 10 min. Proteins were separated by SDS-PAGE, transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore), and then blocked with 5% non-fat milk in TBST (TBS + 0.1% Tween 20) at room temperature for 1.5h. .. The membrane was incubated overnight at 4 °C with primary antibodies, including antiALOX5, sc-136195, santa-cruz; anti-β-Actin, sc-47778, santa curz; anti-Flag, F1408, Sigma; anti-p-JAK1, 74129, CST; anti-pSTAT1, 8826, CST; Membrane:Article Title: Tumoral ALOX5 mediated arachidonic acid metabolism regulates immune response in non-small cell lung cancer Article Snippet: Equal amounts of protein were mixed with loading buffer and heated at 95 °C for 10 min. Proteins were separated by SDS-PAGE, transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore), and then blocked with 5% non-fat milk in TBST (TBS + 0.1% Tween 20) at room temperature for 1.5h. .. The membrane was incubated overnight at 4 °C with primary antibodies, including anti-ALOX5, sc-136195, santa-cruz; anti-β-Actin, sc-47778, santa curz; anti-Flag, F1408, Sigma; anti-p-JAK1, 74129, CST; anti-pSTAT1, 8826, CST; Article Title: MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer Article Snippet: The protein was loaded into the wells of a 12% SDS-PAGE gel (BioFroxx, China) (40 μg/well) and separated at 80 V for 30 minutes, followed by 120 V until the protein was fully resolved. .. The proteins were transferred onto a PVDF membrane, which was then blocked for 15 minutes using a blocking solution and incubated overnight at 4 °C with the following primary antibodies: Anti-pro-caspase-1 (1:1000, ab179515, Abcam, United Kingdom), anti-GSDMD (1:1000, ab210070, Abcam, United Kingdom), anti-cleaved caspase-3 (1:500, ab32042, Abcam, United Kingdom), anti-cleaved caspase-7 (1:1000, ab256469, Abcam, United Kingdom), anti-cleaved caspase-8 (1:1000, #98134, CST, United States), anti-RIPK1 (1:1000, AB300617, Abcam, United Kingdom), anti-p-RIPK1 (1:1000, ab316923, Abcam, United Kingdom), anti-MLKL (1:2000, ab184718, Abcam, United Kingdom), anti-p-MLKL (1:1000, ab187091, Abcam, United Kingdom), anti-GSDME-N (1:1000, ab215191, Abcam, United Kingdom), Article Title: Tumoral ALOX5 mediated arachidonic acid metabolism regulates immune response in non-small cell lung cancer. Article Snippet: Equal amounts of protein were mixed with loading buffer and heated at 95 °C for 10 min. Proteins were separated by SDS-PAGE, transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore), and then blocked with 5% non-fat milk in TBST (TBS + 0.1% Tween 20) at room temperature for 1.5h. .. The membrane was incubated overnight at 4 °C with primary antibodies, including antiALOX5, sc-136195, santa-cruz; anti-β-Actin, sc-47778, santa curz; anti-Flag, F1408, Sigma; anti-p-JAK1, 74129, CST; anti-pSTAT1, 8826, CST; Blocking Assay:Article Title: MiR-200a-3p/ZEB1/IRF1-mediated PANoptosis prompts Xiangshaliujunzi decoction to overcome 5-fluorouracil resistance in gastric cancer Article Snippet: The protein was loaded into the wells of a 12% SDS-PAGE gel (BioFroxx, China) (40 μg/well) and separated at 80 V for 30 minutes, followed by 120 V until the protein was fully resolved. .. The proteins were transferred onto a PVDF membrane, which was then blocked for 15 minutes using a blocking solution and incubated overnight at 4 °C with the following primary antibodies: Anti-pro-caspase-1 (1:1000, ab179515, Abcam, United Kingdom), anti-GSDMD (1:1000, ab210070, Abcam, United Kingdom), anti-cleaved caspase-3 (1:500, ab32042, Abcam, United Kingdom), anti-cleaved caspase-7 (1:1000, ab256469, Abcam, United Kingdom), anti-cleaved caspase-8 (1:1000, #98134, CST, United States), anti-RIPK1 (1:1000, AB300617, Abcam, United Kingdom), anti-p-RIPK1 (1:1000, ab316923, Abcam, United Kingdom), anti-MLKL (1:2000, ab184718, Abcam, United Kingdom), anti-p-MLKL (1:1000, ab187091, Abcam, United Kingdom), anti-GSDME-N (1:1000, ab215191, Abcam, United Kingdom), Activity Assay:Article Title: SLC5A11 Mediates Metformin-Induced PD-L1 Suppression to Enhance Cancer Immunotherapy through AMPK-IRF1 Signaling. Article Snippet: 3 Metformin exhibits immunomodulatory properties in cancer treatment, but the 4 underlying mechanisms remain elusive.. Using genome-wide CRISPR screening, we 5 identified SLC5A11 as an essential mediator of metformin sensitivity.. Molecular 6 docking and dynamics simulations revealed direct metformin-SLC5A11 binding at the 7 pocket containing Asn78 and Glu102 residues. SDS Page:Article Title: Orphan Nuclear Receptors expression and function in breast cancer cells: oncogenic action of the NR2F6 receptor. Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS 0.1%) and quantified with the PierceTM BCA Protein Assay Kit (Invitrogen, ThermoFisher-SCIENTIFIC). .. Protein samples were subjected to SDS-PAGE and Western blot analyses using the following antibodies: anti-NR2F2 (Cell Signaling Technology, #6434), anti-NR2F6 (Proteintech, clone 2H2B8, #60117-2-PBS), anti-p65 Ser536 (NFKB) (Cell Signaling Technology, #3033), anti-p65 (NFKB) (Cell Signaling Technology, #8242), anti-STAT1 Tyr701 (Cell Signaling Technology, #9167), Western Blot:Article Title: Orphan Nuclear Receptors expression and function in breast cancer cells: oncogenic action of the NR2F6 receptor. Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS 0.1%) and quantified with the PierceTM BCA Protein Assay Kit (Invitrogen, ThermoFisher-SCIENTIFIC). .. Protein samples were subjected to SDS-PAGE and Western blot analyses using the following antibodies: anti-NR2F2 (Cell Signaling Technology, #6434), anti-NR2F6 (Proteintech, clone 2H2B8, #60117-2-PBS), anti-p65 Ser536 (NFKB) (Cell Signaling Technology, #3033), anti-p65 (NFKB) (Cell Signaling Technology, #8242), anti-STAT1 Tyr701 (Cell Signaling Technology, #9167), Fluorescence:Article Title: Orphan Nuclear Receptors expression and function in breast cancer cells: oncogenic action of the NR2F6 receptor. Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS 0.1%) and quantified with the PierceTM BCA Protein Assay Kit (Invitrogen, ThermoFisher-SCIENTIFIC). .. Protein samples were subjected to SDS-PAGE and Western blot analyses using the following antibodies: anti-NR2F2 (Cell Signaling Technology, #6434), anti-NR2F6 (Proteintech, clone 2H2B8, #60117-2-PBS), anti-p65 Ser536 (NFKB) (Cell Signaling Technology, #3033), anti-p65 (NFKB) (Cell Signaling Technology, #8242), anti-STAT1 Tyr701 (Cell Signaling Technology, #9167), |